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Introduction
Format
Seminars
Labs
Fees
and
Registration
Accommodation
The Venue
July 20-22
2009 Workshop labs
Provisional schedule
1. FRET - Zeiss Cell
Observer SD spinning disk confocal & MBF Spinning Disk Confocal*
Fixed Mammalian cells expressing a C5V will be used as a
model for quantifying FRET. Images will be acquired to quantify sensitised
emission FRET as well as acceptor photo bleaching FRET.
2.
FRAP -
Olympus FV1000
Cells expressing cytosolic or membrane targeted
fluorescent protein will be used to quantify mobility in the cytosol and in
membranes.
3. Spectral
analysis - Leica TCS-SP5 X white-light confocal &
Leica MP TCS-SP5*
Spectral
separation of similar fluorescent proteins. Fixed cells expressing YFP, GFP or YFP and GFP.
4.
Ratio-imaging
of Biosensors - Ratio-Imaging Workstation* & PTI EasyRatioPro
Dual-wavelength dyes and biosensors are increasing
in number as reporters for dynamic cellular events such as changes in ion
concentration, protein phosphorylation and protease activity.
5. Widefield Deconvolution - Deconvolution Workstation* &
Nikon Ti-E
A comparison will be made using widefield deconvolution and structured
illumination of live yeast expressing GFP and RFP.
6. Image
Processing
and
Analysis with ImageJ - located in the CSU training suite HSC 2D8
A hands-on introduction to ImageJ covering installation, upgrading, plugins and
macros, routine processing and analysis and an introduction to writing macros to
automate and write custom functions.
7. Live cell confocal.
Perkin Elmer Ultraview
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