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Microscopy - Spinning disk confocal

  • Live cell confocal

  • Laser excitation for CFP, GFP, RFP and mPlum

  • Environment control - CO2 and temperature

  • Maximum sensitivity with back thinned EMCCD  camera

  • Up to 30 frames per second

  • Fast piezo Z-drive

System details

Microscope: Leica DMI 6000 B.

Excitation: 440 nm; 491 nm; 561 nm; 650 nm.

Excitation controller: Spectral Applied Research Laser Launch Module LMM5.

Objectives: 20× 0.35NA Ph N-Plan; 40× 0.6NA Ph Fluotar; 63× 1.3NA DIC glyc HCX Pl-Apo.

Camera: Hamamatsu C9100-12 back-thinned EMCCD.

Scanhead: Yokogawa CSU-10.

Emission control: Ludl Mac5000 Controller

Z-controller: Mad City Labs Nanodrive

Environmental control: Neue LiveCell2 whole microscope chamber.

Acquisition Software: Volocity 4

Emission filters:

CY5: Semrock 692/40.

RFP: Semrock 624/40.

YFP: Chroma 545/40.

GFP: Semrock 536/40.

CFP: Chroma. 480/40.

 

 

 

 
   

 

Updated 08/17/2009

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