MacBiophotonics

 

 

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Microscopy - Widefield deconvolution

  • Optimised for single cell immunofluorescence

  • Integrated deconvolution routines

  • Precise piezo z-drive

  • Widefield Time-lapse

System details

Microscope: Leica DMI 6000 B.

Excitation: EXFO metal halide

Objectives: 20× 0.5NA Ph; 40× 1.25NA DIC oil plan apo; 63× 1.4NA DIC oil plan apo; 100× 1.4NA DIC oil plan apo.

Camera: Hamamatsu Orca ER-AG.

Z-controller: Mad City Labs Nanodrive.
Acquisition Software: Volocity 4

 


Widefield image of HeLa cells stained with DAPI (blue), mitotracker green and Lysotracker red overlayed on a DIC image.

Filter sets

Cy5: Semrock 4040A. 628/40; 660 dichroic; 692/40.

RFP: Semrock TRITC-A-000. 543/22; 562 dichroic; 593/40.

YFP: Semrock 2427A. 500/24; 520 dichroic; 542/27.

GFP: Semrock 3035B. 472/30; 495 dichroic; 520/35.

DAPI: Semrock 5060B. 377/50; 409 dichroic; 447/60.

 

Fura2: Chroma D340xv2; D380xv2; 505DCXRU; HQ535/50m.

Ratiometric-Pericam: Chroma D414/30x; HQ485/15x; 505DCXRU; HQ535/50m.

CFP-YFP dual set:

 

Separate excitation filters for DG4.

DAPI: Semrock BrightLine 377/50.

CFP: Semrock BrightLine 427/10.

GFP: Semrock BrightLine 482/35.

YFP: Semrock BrightLine 504/12.

   

 
 

Updated 08/17/2009

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