• Multiphoton excitation

• Capable of transmitted light, red, green, blue and far-red fluorescence imaging (e.g. DIC, FITC, TRITC, DAPI, Cy5)

• Spectral imaging

• Dual channel fluorescence lifetime imaging (FLIM)

• Optimal optical sectioning

System details

Microscope: Leica DMI 6000 B.

Scanner: Leica TCS SP5 with AOBS.

Excitation: 100 mW 488 nm Argon ion; 10 mW 561 nm DPSS; 633 nm HeNe, Chameleon Ultra 690-1040 nm 2.5W at 800 nm.

Objectives: 20× 0.5NA Ph HC-PL; 63× 1.3NA DIC glyc HCX Pl-Apo.

Detector: 2× steady state PMTs; 2× gated-FLIM PMTs.

Software: Leica Application Suite Advanced Fluorescence including:

    FRET AB wizard

    FRET SE wizard

    FRAP wizard